1 VM occurs in watersheds where A. hydrillicola colonizes H. verticillata. Watersheds where VM has been diagnosed (indicated by black crosshatching). Watersheds where H. verticillata has been confirmed to be colonized with A. hydrillicola are shown in red, and watersheds where A. hydrillicola has not yet been observed on H. verticillata are shown in yellow. Watersheds not yet screened for A. hydrillicola, but where H. verticillata occurs, are shown in green. Base map: copyright 2013 from the National Geographic Society. Discovery and production of the putative toxin In 2011, we collected H. verticillata with epiphytic A. hydrillicola from the J. Strom Thurmond Reservoir (in Georgia and South Carolina) to isolate the cyanobacterial strain for mass cultivation and subsequent isolation of the putative cyanotoxin. As a result of challenges in establishing culture conditions for this epiphytic colonizer, it took 2 years to generate sufficient A. hydrillicola biomass for a first feeding trial. The identity of the strain was confirmed as A. hydrillicola by 16S ribosomal RNA sequencing.https://science.sciencemag.org/content/371/6536/eaax9050
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